[Loading Dye with sensitive DNA-Dye NonTox, Ethidium bromide substitute!]
DNA-Dye NonTox´Â EtBr(Ethidium Bromide)À» ´ëüÇÏ´Â °í°¨µµÀÇ ¹«µ¶¼º(Non-toxic) Çü±¤½Ã¾àÀÌ Æ÷ÇÔµÈ loading dyeÀÔ´Ï´Ù.
µ¶ÀÏ AppliChem»çÀÇ DNA-Dye NonTox´Â ÀÌÁß³ª¼± DNA(ds DNA)°ËÃâ½Ã EtBr¸¸ÅÀÇ ¶Ù¾î³ °¨µµ¸¦ °®´Â ¿°»ö½Ã¾àÀÔ´Ï´Ù. 6X DNA Loading BufferÀÇ ÇüÅ·ΠÁ¦°øµÇ´Â DNA-Dye NonTox´Â DNA marker¿Í agarose³ª, polyacrylamide gel¿¡ loading ÇÒ »ùÇÃÀ» ÁغñÇϴµ¥ »ç¿ëµË´Ï´Ù.
DNA-Dye NonTox¿¡´Â ¼¼ °¡Áö tracking dyeÀÎ Bromophenol Blue, Zylene Cyanol FF, Orange G °¡ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù.
DNA-Dye NonTox¸¦ »ç¿ëÇؾßÇÏ´Â ÀÌÀ¯ !
¡Ü ¾ÈÀü¼º (Safe)
µ¹¿¬º¯ÀÌ (¹ß¾ÏÀ¯¹ß)¸¦ ÀÏÀ¸Å°´Â ¹°ÁúÀÌ µé¾îÀÖÁö ¾Ê½À´Ï´Ù. ¶ÇÇÑ µ¶¼ºÀִ ȯ°æÆó±â¹°À» ¸¸µéÁö ¾Ê½À´Ï´Ù.
DNA-Dye NonTox´Â À§ÇèÇÑ Á¦Ç°(Dangerous Good)À¸·Î ºÐ·ùµÇÁö ¾ÊÀ¸¸ç, ¼öÁú¿À¿°¿¡ ¿µÇâÀ» ³¢Ä¡Áö ¾ÊÀ¸¸ç, Æó±â¹° 󸮺ñ¿ëÀÌ ÀüÇô µéÁö ¾Ê½À´Ï´Ù.
¡Ü ¶Ù¾î³ °¨µµ (Sensitive)
EtBr ¸¸ÅÀÇ ¶Ù¾î³ °¨µµ¸¦ º¸¿©ÁÝ´Ï´Ù.
¡Ü DamageÀÇ ÃÖ¼ÒÈ(Mild)
EtBrÀº DNA nickingÀ̳ª strand break¸¦ ÀÏÀ¸Åµ´Ï´Ù. ±×·¯³ª EtBr ´ë½Å DNA-Dye NonTox¸¦ »ç¿ëÇϸé ÀÌ¿Í °°Àº damage¸¦ ÃÖ¼ÒÈ ÇÒ ¼ö ÀÖ½À´Ï´Ù. blue light¸¦ »ç¿ëÇÏ´Â °æ¿ì¿¡´Â DNA¿¡ ¹ÌÄ¥ ¼ö ÀÖ´Â ºÎÁ¤ÀûÀÎ ¿µÇâÀ» ÈξÀ ´õ ÁÙÀÏ ¼ö ÀÖ½À´Ï´Ù. DNA-Dye NonTox¸¦ »ç¿ëÇÑ °æ¿ì EtBrÀ» »ç¿ë ÇßÀ» ¶§ º¸´Ù cloning ½ÇÇè½Ã DNA¿¡ ¹ÌÄ¡´Â damageÀÇ ÃÖ¼ÒÈ·Î ÀÎÇØ Çö󽺹̵å DNAÀÇ ÇöÀúÈ÷ ³ôÀº ÇüÁúÀüȯ¼Óµµ (Transformation rate)¸¦ º¸¿© ÁÖ°Ô µË´Ï´Ù.
¡Ü Ready to use (¹Ù·Î »ç¿ë°¡´É!)
6X loading Dye·Î »ç¿ëÇÏ¸é µÇ°í, staining, de-staining °úÁ¤
ÀÌ ÇÊ¿äÇÏÁö ¾Ê½À´Ï´Ù.
EtBr(Ethidium Bromide)ÀÇ ´ëü !
µ¶ÀÏ AppliChem»çÀÇ DNA-Dye NonTox´Â ¹ß¾ÏÀ¯¹ß°¡´É¼º°ú °ÇÑ µ¶¼ºÀ» Áö´Ï°í ÀÖ´Â EtBr ´ë½Å »ç¿ë °¡´ÉÇϹǷÎ, ½ÇÇèÀÚ¿¡°Ô À¯ÇØ ÇÏÁö ¾ÊÀº(non hazardous) ¾ÈÀüÇÑ ½ÇÇèȯ°æÀ» Á¦°øÇÕ´Ï´Ù.
¶ÇÇÑ, DNA-Dye NonTox¿¡ µé¾îÀÖ´Â ¿°»ö½Ã¾à(dye)Àº DNAÀÇ ±¸Á¶¸¦ Æı«Çϰųª º¯Çü½ÃÅ°Áö ¾ÊÀ¸¸é¼µµ EtBr¸¸ÅÀÇ ¶Ù¾î³ °¨µµ¸¦ ÀÚ¶ûÇÕ´Ï´Ù.
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1. »ç¿ëÀü¿¡ 10ÃÊ µ¿¾È DNA-Dye NonTox¸¦ Vortex Çϼ¼¿ä.
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2. DNA »ùÇðú DNA-Dye NonTox´Â 5:1·Î Èñ¼®ÇÕ´Ï´Ù.
(* Note : DNA-Dye NonTox´Â DNA marker¿¡µµ ³Ö¾îÁÖ¾î¾ß ÇÕ´Ï´Ù.)
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3. »ùÇÃÀ» loading ÇÏ°í Àü±â¿µµ¿À» ¼öÇàÇÕ´Ï´Ù.
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4. Àü±â¿µµ¿ ÈÄ, UV³ª Blue light transilluminator·Î È®ÀÎÇÕ´Ï´Ù.
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<º¸°ü> 2-8¡É, ºûÀÌ ´êÁö ¾Ê´Â °÷ <À¯È¿±â°£> 12°³¿ù
Tracking Dyes included : Bromophenol blue, Xylene cyanol FF, and Orange G
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Ordering Information
Product Name
|
Order No.
|
Quantity
|
DNA-Dye NonTox
|
A9555.1000 |
1 ml |